Purification of rat liver microsomal cytochrome P-450 by chromatography on immobilized adamantane with a new method of detergent removal.
نویسندگان
چکیده
A method for the isolation of liver microsomal cytochrome P-450 from phenobarbital-treated rats is described which is based on chromatography on 1-adamantane-carbonyl-aminohexyl-Sepharose 4B, subsequent ion exchange chromatography on DEAE-cellulose and detergent removal by adsorption on silica gel G. The method gives preparations with high electrophoretic homogeneity which correspond to a major phenobarbital-inducible form of rat liver microsomal cytochrome P-450. The advantages of the procedure described are high yields, stability of chromatographic material, effective and simple method of detergent removal.
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عنوان ژورنال:
- Biomedica biochimica acta
دوره 43 12 شماره
صفحات -
تاریخ انتشار 1984